Pre-Treatment of Raw Sugarcane Molasses by Metal Complexing Agents for Improved Citric Acid Fermentation by Aspergillus Niger
نویسندگان
چکیده
The present study deals with the pre-treatment of sugar cane molasses for the enhanced production of citric acid by Aspergillus niger NG-4. Different metal complexing agents such as potassium ferrocyanide [K4Fe(CN)6] and ethylene diamine tetra acetic acid (EDTA) were also employed for molasses pre-treatment and the concentrations varied from 50-350 ppm. The maximum production of citric acid (36.4 g/l) was achieved in the medium pre-treated with 250 ppm potassium ferrocyanide. The kinetic parameters such as growth yield coefficients (Yp/s, Yp/x, Yx/s in g/g), volumetric rates (Qp, Qs, Qx in g/l/h) and specific substrate rates (qp, qs in g/g cells/h) of the research work were also undertaken. The value of Qp (0.134 g/l/h) is highly encouraging (p≤0.05).
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